GE Healthcare, Percoll, 250ml, 17-0891-02 is a well-referenced media for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 A- g). Percoll is supplied sterile and is easily resterilizable. The medium is avaialble in easy-to-open, resealable 250 ml and 1 l bottles.Percoll consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll is non-toxic, almost chemically inert and does not adhere to membranes. Percoll gradients can be formed within the density range of 1.0 to 1.3 g/ml, and are iso-osmotic throughout. Percoll is provided as a sterile solution and can be stored unopened at room temperature for five y. At -20°C, it can only be stored for up to six months. If stored at -20°C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient is sterile and remains unfrozen. Percoll can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures. Undiluted Percoll can be resterilized by autoclaving for 30 min at 120°C.GE Healthcare, Percoll, 250ML 17-0891-02 / 95038-164 is part of a wide selection of GE Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction. Combining excellent quality with an affordable price, the GE Percoll 250ML 17-0891-02 / 95038-164 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction please do not hesitate to contact our customer service for further guidance.
PERCOLL 250ML BOTTLE.