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GE Prescission Protease 27-0843-01
$429.99
Code: Y2-VW-27-0843-01
MPN: 27-0843-01
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GE Healthcare, PreScission Protease, 27-0843-01 Product Info

GE Healthcare, PreSscission Protease, 27-0843-01 PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. This protease was specifically designed to facilitate removal of the protease by allowing simultaneous protease immobilization and cleavage of GST fusion proteins produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3; see pGEX Vectors (GST Gene Fusion System). PreScission Protease specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro. GE Healthcare, PreScission Protease, 27-0843-01 / 95017-584 is partimage of a wide selection of GE Antibodies. Combining excellent quality with an affordable price, the GE Prescission Protease 27-0843-01 / 95017-584 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Antibodies please do not hesitate to contact our Laboratory Product Specialists for further guidance.

PreScission Protease. For site-specific separation of the GST affinity tag from proteins expressed using pGEX-6P vectors.Enables the low-temperature cleavage of fusion proteins containing the PreScission* Protease recognition sequence.

Specifications for GE Healthcare, PreScission Protease 27-0843-01:

Complete Packsize: 500 units
Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors
Unit Definition: One unit will cleave > 90% of 100 μg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h.
Vector: pGEX-6P-1, pGEX-6P-2, pGEX-6P-3
Molecular Weight: Mr 46000
Inhibitor: 100 mM ZnCl2 (< 50% inhibition); 100 μM chymostatin; 4 mM Pefabloc
Elution Buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Cleavage Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0
Supplied As: 2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol.

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