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Microscope for a sperm study

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Microscope for a sperm study

Postby deetrin on Wed Apr 04, 2007 10:12 am

I am doing a study of sperm viability post vasectomy and I need to know what strenth and type of microscope would be adequate for this purpose. Can you also recommend an inexpensive model(s)that would work for that purpose. Thank you.
deetrin
 

Postby Jne_K on Wed Apr 04, 2007 10:13 am

Hi

You need a phase contrast microscope to study such trnaparent specimens. This is the standard type of microscope for sperm counts. At the moment, we do not offer an inexpensive phase contrast scope, but when available, the LOMO MULTISCOPE TMH4-BF-V Microscope with adapter for Video & Photomicrography (VA-VAV2 C-Mount Adapter for HT-30) is one of the least expensive models on the market. Price start at about $900.
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I beg to differ

Postby Scopemaster on Wed Nov 05, 2008 1:35 pm

Jne_K, if all you want to do is check for motility (movement), all you need is 400x. Yes, phase contrast is best for morphology, but it sounds like all you want to do is make sure they are dead, and that can be done with an inexpensive student microscope. One with an iris diaphragm would be perferable, but a disk diaphragm will do in a pinch as well.
Scopemaster
 

Postby Jne_K on Thu Nov 06, 2008 6:53 am

Hi

Good point.
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Forum: http://www.opticsplanet.com/msgboard
Blog: http://blog.opticsplanet.com/
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Phone: (800) 504-5897
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