Corning Molecular Biology Buffers and Reagents, Corning 46-034-CI Edta Solution, 0.5 M, Ph 8.0 22% Off w/ Free S&H

With Ethylene Diamine Tetraacetic Acid (EDTA), one of the major reducing agents in organic acid fermentations, ethyl acetate is an effective reducing agent for reducing a wide variety of organic acids. The 0.5 M sodium EDTA concentration in this solution reduces the solubility of proteins and decreases the solubility of organic acids. Unlike most organic acids, EDTA does not react with ethyl acetate. Generally considered to be a safer alternative to formal NaOH EDTA in organic solvent systems, reducing agents in EDTA may cause denaturation of the labeled amino acids. A reaction with an amino acid sequence similar to that of the nucleic acids will produce a precipitate in an organic acid solution. Nitrogen is incorporated in very small amounts into the molecular level to prevent denaturation of DNA. Its presence in the reduced form in most molecular biology buffers and reducible acids reduces the amount of DNA that remains in the sample. This decrease in DNA binding reduces the amount of DNA that is lost during electrophoresis.

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