Shop now and get Free Value Shipping on most orders over $49 to the
contiguous 48 states, DC, and to all U.S. Military APO/FPO/DPO addresses.
Enjoy our FREE RETURNS
We want to ensure that making a return is as easy and hassle-free as possible! If for any
reason you are not satisfied with your purchase, simply return the item within 30 days of
receipt, and we'll cover the cost of return shipping. Learn more about our Return Policy.
Please Enable Your Browser's Cookies Functionality
Cookies are not currently enabled in your browser, and due to this the functionality of our site will be severely restricted.
Web browser based cookies allow us to customize our site for you, save items in your cart,
and provide you with a great experience when shopping OpticsPlanet.
Your privacy is important to us, and any personal information you supply to us is kept strictly confidential.
If you cannot enable cookies in your browser, please contact us — we are always here to help!
JavaScript is blocked by AdBlocker or ScriptBlocker
We're sorry - it looks like some elements of OpticsPlanet are being disabled by your AdBlocker.
Unfortunately we are unable to offer our excellent shopping experience without JavaScript.
Please add "opticsplanet.com" and "https://opl.0ps.us/assets-5239c9a18bf/" to whitelist,
or disable AdBlocker for this site (please note that we do NOT feature any annoying ads on this website).
Thanks!
Your Browser is Outdated
Unfortunately, we are unable to provide an excellent shopping experience on your browser because it lacks modern functionality needed for us
to provide a fast, secure, and enjoyable experience. If you cannot upgrade your browser or use an alternative device to visit us,
please contact us at +1-800-504-5897 and we'll be happy to assist you over the phone!
Effect of Buffer on HPLC Separation of Buffers
— Page 1
HPLC Separation of Buffers'>
Application Notes:
Application Columns:
Obelisc N columns are the first commercially available columns with Liquid Separation Cell technology (LiSC). With multiple patents pending, LiSC technology is based on a new chemical modification of silica gel pores that creates a liquid separation cell with its own charge characteristics, ionic strength, and hydrophobic properties. Like living cells which exist in equilibrium with the outside environment, liquid separation cells exist in constant equilibrium with the mobile phase. Obelisc N, for normal phase, has very polar characteristics and works well for polar and charged analytes. In ion-exchange mode, charged analytes interact with oppositely charged groups on the stationary phase. In traditional HILIC mode, a charged or neutral polar analyte interacts with a water layer on the polar stationary phase surface. On Obelisc N the charges are greatly separated and independently accessible which results in different selectivity compared to traditional HILIC and silica columns. Mobile phase composition changes the conformation of the long hydrophilic chain. This affects the properties of the liquid separation cell and changes separation selectivity. Typical mobile phases used with Obelisc N columns are based on acetonitrile, water, and the mass spec compatible buffers ammonium formate (pH 3) and ammonium acetate (pH 5). If it is necessary to detect in low UV (<220 nm) then phosphate buffer is recommended. Obelisc N columns are compatible with mass spectrometers (LC/MS), evaporative light scattering detectors and preparative chromatography (prep HPLC) system.
Your Browser is Outdated
HPLC Separation of Buffers'>